MetaXpress® 5.0 high-content image acquisition and analysis software has been designed to improve the ease and speed of custom analysis using ImageXpress® Micro and Ultra systems. The new software enables user-created analysis to be run on MetaXpress PowerCore™ software, which can reduce analysis time from hours to minutes. An interactive tool incorporated into the software allows users to create custom analysis without resorting to dedicated programming expertise. The system includes a total of 19 predesigned analysis routines, with the facility to add in custom steps to create a tailored analysis. MetaXpress 5.0 also features object click-to-find, allowing scientists to identify objects of interest within their images.