EnPresso Y Defined is a high cell-density medium growth system for improved cell growth and increased protein expression in Pichia pastoris with methanol induction. The EnPresso Y Defined tablet is a presterilized medium, which employs the patented EnBase technology to control the enzymatic release of glucose. The EnPresso Y defined-growth system controls cell growth, maintains favorable pH, can improve the productive yield, and allows optimization prior to scale-up.