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March 1, 2012 (Vol. 32 , No. 5)

<i>P. pastoris</i> Protein Expression

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DNA 2.0

Synthetic genes are available in vectors that are immediately usable for P. pastoris protein expression. Cloning into these vectors is quickly achieved and the constructs are provided without IP restrictions. pJexpress Pichia features resistance marker choice: Zeocin (selection in both E. coli and P. pastoris) or Kanamycin (E. coli)/Geneticin (P. pastoris). An alpha factor signal sequence is available for secreted expression. Users have a promoter choice of AOX1 for methanol inducible expression or GAP1 for constitutive protein expression. Vectors are recombined into chromosome using SwaI (or SacI) and BamHI. Recombination within the chromosome is not localized.

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