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September 1, 2012 (Vol. 32 , No. 15)

High-Throughput ChIP

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EMD Millipore

Using the Magna ChIP™ HT96 kit, high-throughput chromatin immunoprecipitation (ChIP) can be performed on up to 96 samples in a single experiment using as few as 10,000 cells per well. The protocol uses a single buffer for sonication, immunoprecipitation, and washing, which can yield good sensitivity and low backgrounds. The Magna ChIP HT96 protocol has been designed for immunoprecipitation and recovery of DNA; in many cases, a separate DNA purification step is not required for downstream analysis.

  • EMD Millipore
  • 290 Concord Road
  • Billerica MA, 01821
  • UNITED STATES

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