The acumen® hci (high-content imaging) can image and analyze the whole wells of 96- to 1,536-well plates at a resolution of 0.5 µm/pixel. This enables the screening of high-density plates and can facilitate assay miniaturization. It also incorporates cellista software, which can enable high-throughput whole-well imaging of a range of assays including angiogenesis, neurite outgrowth, nuclear localization, translocation, and cytotoxicity. The open-source, OME-compliant TIFF images can be exported and imported into a range of open source and commercial image analysis packages. The acumen hci is also compatible with microscope slides using the widefield objective for imaging of whole tissue sections.