The scan^R 2.4 high-content screening station is based upon the IX3 inverted microscope frame. The updated screening station can now be incorporated with the fully automated IX83, combining a modular frame design with scan speed. This new system can deliver quantitative expert results in a range of high-content screening of cell-based assays, from gene expression to bacterial infection assays. The scan^R 2.4 software capabilities have also been extended, including multicore and kinetic analysis for advanced microscopy techniques. The scan^R 2.4 station is designed for many different assay formats, including multiwell plates, slides, and custom-built arrays.