96-well Perfecta3D® hanging-drop plates help researchers to form and test reproducible 3D cell cultures. The design of the Perfecta3D hanging-drop plates can simplify and streamline spheroid formation, culture, and subsequent testing of the 3D cellular constructs without the aid of coatings or matrices. Spheroid cultures grown in Perfecta3D hanging-drop plates can allow researchers to mimic tissue metabolic and proliferative gradients, capture complex cell-matrix and cell-cell interactions, conduct co-cultures, and monitor cell growth. Media and compounds can be added or removed from the top of the plate without requiring new equipment, and spheroids can be harvested for analysis.