The Mission Target ID Library enables benchtop transcriptome-wide human miRNA and ncRNA gene target identification. The Target ID Library is a transcriptome-wide cDNA pool; the library’s plasmids each have a human cDNA inserted into the 3´-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing. The library is provided in ready-to-use DNA format, with enough material for approximately 6 to 10 genome-wide screens.