Exome Analysis helps simplify the processing and investigation of exome datasets and allows users to determine whether regions of interest have been sequenced with sufficient coverage. Results are reported as the number and fraction of bases covered by sequence reads, along with average coverage within an exon. Users can then perform further analyses using the Nucleotide-Level Variation or the Cancer Variation analyses, which compare, for example, a cancer tissue sample and a normal sample. With support for user-uploaded targets and exome kits from Agilent and NimbleGen, this application is accessible via a Cloud-based platform.