SimpleStep kits are single-wash, colorimetric sandwich ELISAs that are designed to retain the familiar ELISA process and data outputs. Designed for users who may lack specialized training or instrumentation, SimpleStep kits can be used with fewer sample handling steps. The kits are available in off-the-shelf 1, 2, or 4 target conformation. Custom multitarget kits are also available, allowing customers to select analyte combinations. Potential applications for SimpleStep kits include the investigation of intra- or extracellular signaling pathways and cancer biomarker research.