Accel-NGS 2S DNA Library Kit for Illumina next-generation sequencing (NGS) systems provides linear yields from inputs ranging from 10 pg to 1 µg. PCR-free libraries can be produced from as little as 100 ng. The product can also be used for clinical samples such as FFPE tissues and plasma. Accel-NGS 2S can enable NGS laboratories to use a single kit for varied DNA library needs, ranging from high-molecular-weight DNA to limited quantity, damaged FFPE samples. In addition to whole-genome sequencing, Accel-NGS 2S is also compatible with leading hybridization capture products and is suitable for ChIP-Seq. The 2S protocol is designed to be automatable for those with high-throughput applications.