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May 1, 2012 (Vol. 32 , No. 9)

DNA Fragment Assembly

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New England BioLabs

Gibson Assembly uses a one-step, isothermal approach to enable the rapid assembly of multiple DNA fragments. Developed in partnership with Synthetic Genomics, the Master Mix is optimized for assembling three or more fragments at near 100% efficiency. The Gibson Assembly Master Mix includes protocols for primer design, and can be used for assembly of multiple DNA fragments, including linearized vector and insert. It can be used by researchers to assemble such synthetic nucleic acids into larger genetic constructs.

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