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September 1, 2013 (Vol. 33 , No. 15)

DNA Enrichment Kit

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New England BioLabs

The NEBNext® Microbiome DNA enrichment kit can separate microbial DNA from host DNA. The kit utilizes the MBD2-Fc protein, which can bind to CpG-methylated DNA (including human genomic DNA) with high specificity. The MDB2-Fc protein is attached to Protein A-coated magnetic beads, allowing for the removal of the contaminating host DNA in about 30 minutes. The microbial DNA-enriched sample is then ready to be processed for multiple downstream applications, including next-generation sequencing, qPCR, and endpoint PCR.

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