HiTrap™ Talon® crude, His SpinTrap™ Talon microspin, His MultiTrap™ Talon, and His GraviTrap™ Talon are prepacked with Talon Superflow™ media for screening, preparative purification, and scale-up of histidine-tagged proteins. Samples derived from a range of expression systems can be purified with reduced sample-preparation time. Talon Superflow is a cobalt-based medium designed for the purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography. Cobalt-based media can aid in selectivity and yield purity; additionally, lower concentrations of imidazole are required during purification, which can help preserve the structure and function of sensitive histidine-tagged proteins.