MoFlo Astrios next-generation cell sorter leverages the stable fluidic design of the original MoFlo and the electronic processing of the MoFlo XDP, while expanding sorting capabilities with an array of laser options. It features beadless drop delay determination, and a range of software components help to further enhance instrument functionality. Complex, multicolor sorting is enabled by seven pinholes that spatially separate each of the seven lasers. Auto start-up allows the researcher to define when lasers and fluidics turn on. The multifiber Beam Shaping Optic creates flat-top beam profiles, providing short alignment times and high-level optic stability. Two stages control the alignment for all seven lasers to help simplify sorter preparation.