Adaptable to standard instrumentation, these cell-signaling assays directly measures kinase, phosphatase, phosphodiesterase, and protease activity. They can be scaled and automated for high-throughput screening. Useful for biochemical analysis, cell-based analysis, endpoint monitoring, kinetic monitoring, and other analyses, the assays produce stable detection products in approximately 24 hours. They require no radioactivity or antibodies and are available for PKA, P13K, Sphingosine kinase, Fyn kinase, PTP1B, PDE1C, PDE4A1A, and PDE5A. Also offered is a PanUmbra assay that allows researchers to develop custom assays for particular enzymes of interest.