The Cultrex® 3D cell invasion assay uses a 3D culture-qualified 96-well plate with a spheroid formation ECM to drive aggregation and/or spheroid formation of cells. Upon completion of formation, the spheroid is embedded in an invasion matrix composed of basement membrane proteins. This matrix forms a hydrogel network through which invasive cells can travel. Invasion-modulating agents can then be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantitated through image analysis software.