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April 1, 2014 (Vol. 34 , No. 7)

BRCA1 and BRCA2 Panel

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WaferGen Biosystems

The Seq-Ready™ TE BRCA 1/2 Panel contains 139 unique PCR primer pairs that can target 100% of the coding regions of BRCA1 and BRCA2 tumor suppressor genes. Designed and verified with two leading clinical research laboratories, the panel was tested against hundreds of samples, reportedly yielding 100% concordance with Sanger sequencing. All known mutations were detected for all exons covered, obtaining an overall average coverage of greater than 100x with 24 samples on a MiSeq® (2 x 150 bp). Seq-Ready TE technology, which relies on massively parallel singleplex PCR, is designed to both enrich a large number of target regions and also incorporate sequencing adapters and barcodes to produce sequencing-ready libraries in a single step.

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