The TriPlus 300 headspace autosampler has been designed for analyzing organic volatiles. This valve-and-loop headspace autosampler features a 120-vial sample tray capacity and 18-vial incubation oven overlap capacity, enabling unattended high-throughput runs. After initial setup, the device’s timing automatically maintains constant equilibrium intervals. Sample integrity is protected and carry-over effects are mitigated because the entire sample path of the TriPlus 300 headspace autosampler is chemically inert, and all heated zones of the headspace unit can be set between 30°C and 300°C. This autosampler solution is compatible with standard GC and GC-MS systems including those using packed and capillary columns.