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February 15, 2012 (Vol. 32 , No. 4)

Autophagy Quantification

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Life Technologies

Premo™ Autophagy Terbium/GFP TR-FRET LC3B kits allow users to see and quantify autophagy. Autophagy activity is measured using cells expressing GFP-tagged LC3B combined with a Terbium (Tb)-based TR-FRET immunoassay approach. This format provides an objective autophagy measurement. The assay utilizes a 96- or 384-well format, allowing speed and scalability not available with traditional autophagy methods such as Western blot, fluorescence microscopy, and high-content analysis.

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