The Screen-Well™ Autophagy compound library is a focused collection of autophagic compounds for cell-based and biochemical applications. It is a tool for studying the roles of pro- and anti-autophagic molecules in cells and is for use in in vitro applications. A variety of structurally and mechanistically different compound classes are included. The library contains 96 compounds with defined autophagy-inducing or autophagy-inhibiting activity. The Screen-Well Autophagy Compound Library is supplied in biocompatible solvents, and no reconstitution is needed before use. Individual compounds of the library are available for resupply.