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July 1, 2014 (Vol. 34 , No. 13)

Assays for Mutation Detection

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Bio-Rad Laboratories

The Droplet Digital PCR (ddPCR™) assays are predesigned and fully wet-lab validated for digital PCR, which frees researchers from time-consuming and labor-intensive assay design and experiment optimization. The ddPCR technology provides an absolute measure of target DNA molecules and, together with PrimePCR mutation detection assays, can enable detection of one mutant molecule in a background of 2,000 wild-type molecules in a single well without the need for a standard curve. In addition, the high precision and absolute quantification made possible by PrimePCR ddPCR assays enable the quantitative discrimination required to resolve small fold changes in gene copy numbers for CNV analysis. PrimePCR digital PCR assays are available in multiple reaction sizes and are compatible with both the QX100 and QX200 ddPCR systems using the ddPCR supermix for probes.

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