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August 1, 2011 (Vol. 31 , No. 14)

Antibody Purification

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GE Healthcare Life Sciences

The rProtein A GraviTrap™, Protein G GraviTrap, and rProtein A/Protein G GraviTrap gravity-flow columns enable single-step manual purification of monoclonal and polyclonal antibodies from various species in milligram scale. The purification run time is approximately 30 minutes (dependent on sample). Designed for small-scale antibody purification, the product package contains everything needed for manual purification, as it converts into a column stand (Workmate) with a plastic tray to collect liquid waste. Each pack contains 10 prepacked columns to facilitate multiple samples to be purified simultaneously, using a gravity format. Columns contain 1 mL bed volume and can bind milligram amounts of antibodies. The columns are reusable up to five times depending on the sample.

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