The Evolute® Express family of 96-well plates can simplify polymer-based solid phase extraction (SPE) by employing a streamlined three-step protocol: load, wash, elute. By using this approach and removing the conditioning and equilibration steps necessary with traditional bioanalytical SPE procedures, processing and method development times can be reduced without loss of analyte recovery or method robustness. The Evolute Express design provides a uniform flow in the processing of aqueous samples and reduces the need to rerun samples due to well-clogging.