Related to Scientists Develop Nanoliter System for Prolonged Single-Cell Imaging of ESC Proliferation

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• Pre-Coated Well Plates
• Trevigen
• The 3D Culture Matrix-coated 96-well plate is coated with a reconstituted basement membrane matrix to provide an environment that is common for epithelial or endothelial cell types. This plate can provide a physiologically predictive format for eva
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• Stem Cell Lines
• Genea Stem Cells
• Embryonic pluripotent cell lines are available including one disease free pluripotent cell line and 24 others with individual mutations that can give rise to several severe diseases such as cancer (breast cancer, Wilm’s tumor, and Von Hippel–Lindau
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• In Vivo Imaging
• PerkinElmer
• The IVIS Lumina series III benchtop imaging system integrates bioluminescence and full spectral fluorescence in in vivo imaging technologies. The platform can perform multiple modalities including photographic, bioluminescent, fluorescent imaging,
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• 3L Fernbach Culture Flask
• TriForest Enterprises
• Autoclavable 3L Fernbach flasks are now available. They feature increased surface area-to-volume ratio to provide greater air and media circulation. The flask is manufactured with FDA-approved Lexan® polycarbonate resin to offer glass-like opt
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• Microplate Detection Platform
• Molecular Devices
• The SpectraMax i3 platform’s base system features an integrated optical system enabling top and bottom reads for 6–384-well microplates and launches with three detection modes: luminescence, absorbance, and fluorescence. The user-exchangeable cartr
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• Temperature-Controlled Shaker
• Benchmark Scientific
• The MultiTherm shaker is a temperature-controlled vortexer with interchangeable blocks for tubes from 0.2 mL to 50 mL. It has a small, 8x11-inch footprint and programmable speed, time, and temperature settings. Temperature control from 0º to 1
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• Image Analysis System
• Syngene
• The PXi Touch comes as a compact, ready-to-use unit with a built-in touch screen and processor. Each PXi Touch features a camera with a large fixed aperture lens, which enables scientists to capture images of faint bands or spots. The system is ava
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• 3D Cell Culture Kits
• Midsci
• Available through an agreement with n3D Biosciences, Bio-Assembler kits utilize magnetic levitation to culture cells in 3D. Available in single, six, and 24-well configurations, the kits supply magnetic drivers, low-binding dishes/plates, and Nanos
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• Fluorescent Protein
• Allele Biotech
• mNeonGreen is a monomeric green fluorescent protein derived from the lancelet (cephalochordate) Branchiostoma lanceolatum . It has been demonstrated to perform as a fusion tag to a large number of target proteins when expressed in mammalian cells.
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• CCD Cameras
• Quantum Scientific Imaging
• The QSI RS series features a range of full-frame and interline transfer CCDs from 768x512 pixels up to 8.3MP. Dual-read rates of up to 8 MHz with USB 2.0, deep cooling to greater than 45°C below ambient and 16-bit pixel depth allow QSI RS seri
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• Nanoparticle Viewing Unit
• NanoSight
• The NS300 utilizes particle characterization technology and can visualize and measure particles in suspension in terms of size, light scattering intensity, fluorescence, and count. It does so particle-by-particle in the range 10–1,000 nm (material
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• In Vivo Imaging Agent
• PerkinElmer
• The GFR-Vivo™ 680 imaging agent allows researchers to measure glomerular filtration rate noninvasively, in vivo in animals. GFR-Vivo 680, in combination with fluorescence molecular tomography imaging, can provide a noninvasive approach to generate
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• RNA Detection Probes
• EMD Millipore
• SmartFlare™ probes employ inert nanoparticle technology to detect native RNA. The probes are added to cell cultures and incubated overnight. RNA detection is then performed using any fluorescent detection platform. Once an experiment is complete, t
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• 24-Well 3D Cell Culture Kit
• TAP Biosystems
• The RAFT 24-well kit has been developed in response to researchers’ requests for a 24-well format to generate single or multiple collagen-based 3D cell culture models. The kit is packaged individually to give flexibility in producing the number of
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• Stem Cell Medium
• Life Technologies
• Essential 6™ medium is designed for reprogramming, expanding, and differentiating pluripotent stem cells. Along with Essential 8™ media, it can streamline the generation, expansion, and differentiation of pluripotent stem cells by offering a feeder
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• Apoptosis-Measuring Reagent
• Essen BioScience
• The CellPlayer Caspase-3/7 apoptosis reagent is designed to fluoresce upon activation of Caspase-3/7 in living cells. This mix-and-read reagent can be multiplexed with measurements of proliferation to differentiate inhibition of proliferation from
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• Microbial Picker
• Hudson Robotics
• The RapidPick Harvester™ is an automated picker for microbial and fungal samples in microplates, omnitrays, and petri dishes. It extracts sample plugs from agar or liquid media. Samples are identified via direct camera image or from output files of
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• Stem Cell Culture Medium
• STEMCELL Technologies
• TeSR™-E8™ is a feeder-free culture medium for human embryonic stem cells (ES cells) and human induced pluripotent stem cells (iPS cells). TeSR-E8 contains only the essential eight components required for maintenance of ES cells and iPS cells. TeSR-
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• Cell Culture Dishes
• Sarstedt
• lumox® cell culture dishes with ultra-thin film bases are only 25 µm thick and provide good optical quality, very low autofluorescence, and high UV transparency for microscopy and imaging. The lumox film is gas-permeable for gas exchange
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• Hematopoietic Colony Imaging System
• STEMCELL Technologies
• STEMvision™ is a bench-top instrument and computer system designed specifically for automated imaging and counting of hematopoietic colonies in the colony-forming unit assay. This system is meant to be used with MethoCult™ media and meniscus-free S