Related to Improving Protein Yields in Mammalian Cells

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• Rat Transgene Integration
• Sigma-Aldrich
• The CompoZr® Targeted Integration Kit-rRosa26 integrates transgenes into the Rosa26 safe harbor locus in the rat genome. CompoZr targeted integration kits employ CompoZr zinc finger nucleases (ZFNs) for precise and heritable gene knock-in, ena
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• Bioprocess Monitoring System
• Stratophase
• The Ranger™ system can be integrated into all bioprocessing systems running microbial or mammalian cell cultures, and enables bioprocess managers to monitor and control metabolic rate, observe batch processing trends, and determine end of process.
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• Hybrid Connector
• Colder Products
• The AseptiQuik® STC for use in biopharmaceutical processes uses a hybrid combination of stainless equipment and single-use systems. The combined AseptiQuik sterile connector and Steam-Thru® II SIP connector can give biopharmaceutical manu
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• Bioprocess Performance Troubleshooting
• Spinnovation Biologics
• Spedia-Predict™ monitors and validates process-critical parameters to help manage the culture media consistency used in a production environment and can help to prevent failure and performance variability in large-scale commercial production. Spedi
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• Sterile Sampling Bottles
• EMD Millipore
• The NovaSeptum® 125, 250, and 500 mL bottles are designed for sterile sampling from biomanufacturing processes. The bottles feature a single-piece cap with an O-ring component for container closure. This design can protect product integrity an
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• CHO Cell Cloning
• STEMCELL Technologies
• The ClonaCell™-CHO ACF supplement improves cloning efficiency of CHO cells during cell-line development. The challenges of low cloning efficiency can result in significant waste of time and resources during single-cell cloning. The ClonaCell-CHO AC
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• Peristaltic Pumps
• Watson-Marlow Pumps Group
• Profibus-enabled peristaltic pumps are designed for process monitoring in validated cGMP production. The Bp pump brings Profibus networking to peristaltic pumping; customers can integrate this seal-less, valve-less pump technology into their Profib
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• Pressure Gauge
• Ashcroft
• The DG25 digital pressure gauge provides a 5 full-digit LCD in ranges up to 25,000 psi. Available in accuracies of 0.5% and 0.25% FS, this design boasts a minimum battery life of 2,000 hours. Standard features include an IP67 enclosure, selectable
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• Polyethylene Bottom-Entry Mixer
• Terracon
• A new bottom-entry nonmetallic mixing tank is now available and is being offered in capacities up to 1,000 liters. All models include a cylindrical, cone-bottom molded polyethylene tank for ease of cleaning and complete drainage, as well as a magne
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• Cell-Line Customization Service
• Cellectis bioresearch
• Gene customization experts can modify cell lines at any gene, at any position. Services available include gene modification, knock-out, and targeted transgene integration. Thirteen years of genome customization expertise, TALEN™ technology, and IP
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• Microcarriers for Bioprocess Scale-Up
• Corning B.V. Life Sciences
• A new line of microcarriers for large-scale vaccine and cell therapy manufacturing is now available. The microcarriers can simplify the scale-up of attachment-dependent cells by providing a premeasured, sterile, ready-to-use product for direct tran
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• Dendritic Cell Generation
• HumanZyme
• The HumanKine G4 dendritic cell generation kit can yield DCs from peripheral blood mononuclear cells with no medium changes. This protocol is enabled by HumanKine GM-CSF and HumanKine IL-4, which are included in the kit and produced in a human cell
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• Bioprocess Capsules
• Pall Life Sciences
• SUPRAcap™ 50 capsules are designed to help you choose the right process-volume depth filter media. They are scalable to SUPRAcap 100 capsules or SUPRAdisc™ I/SUPRAdisc II depth filter modules and are also available with Seitz® HP sheet media (
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• Membrane Filter Cartridges
• Pall Life Sciences
• Supor® EX grade ECV filters are high-capacity, high-flow rate sterilizing-grade filters designed for filtration of a range of liquids in bioprocessing. They feature an asymmetric polyethersulfone (PES) membrane layer that makes them good for f
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• Transfection Reagent
• Mirus Bio
• TransIT®-BrCa transfection reagent is a dedicated breast cancer cell-line specific reagent that can help researchers achieve high expression levels in breast cancer cell types including MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468, and T47D. It c
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• Virus Removal
• Asahi Kasei Bioprocess
• Designed for pilot-, clinical-, and commercial-scale manufacturing of biopharmaceuticals, the Planova™ Virus Filtration Controller can perform consistent virus removal using Planova and Planova BioEX filters. Customary virus filtration operations c
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• Single-Use TFF System
• Pall Life Sciences
• The Allegro™ single-use tangential flow filtration system is fully automated for control and monitoring of critical process parameters. With a fully disposable flow path, the system can help reduce cleaning, downtime, validation, and the risk of cr
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• Single-Use Mixing Platform
• ATMI LifeSciences
• The LevMixer 3 model builds on the second generation LevMixer’s mobile, single-use mixing platform to enable mixing in a range of volumes from 6 L to 2,000 L with real-time reading of impeller speed and a process monitoring/alert capability. The re
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• Aseptic Disconnector
• Sartorius Stedim Biotech
• The Clipster® aseptic disconnector, which is used after a fluid transfer to disconnect transfer lines and bag assemblies, is now available in new sizes XS and S. This single-use device allows an aseptic disconnection of silicone as well as the
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• iPS Cell Lines
• Cellectis bioresearch
• The iPS Engineering Hub™ service can provide users with customized iPS cell lines engineered with TALEN™ technology. Donated somatic cells are reprogrammed by means of co-expressing four factors (Oct-4, SOX2, c-Myc, and Klf-4) by nonintegrating tec