Related to Meeting the Bioassay Challenge

• LAVAgel for Protein Diagnostics
  Researchers introduce a microfluidic, single-channel, multistage immunoblotting strategy toward scalable protein isoform analysis.
  
• Accessible TALENs
  The FLASH system is a rapid and cost-effective method for large-scale assembly of transcription activator-like effector nucleases.
  
• Inhibitors That Put A Kink in Abl's Helix
  Researchers develop a direct-binding assay that enables the detection of allosteric inhibitors, which can suppress a cellular enzyme that contributes to leukemia.
  
• Synthetic Degrons Play The N-End Game
  The Boc₃Arg moiety may provide a general strategy to construct inhibitors that induce targeted protein degradation.
  
• A Faster KAYAK: Mass Spectroscopy for Multiplexed Kinase Assays
  Researchers developed a new, simplified method for measuring the phosphorylation rates of peptide substrates.
  
• Literature Review: BioMAP—Antibiotic Fingerprints
  Researchers show that bioactivity fingerprinting can help with profiling antibiotic lead compounds.
  
• Literature Review: A Flying Drop Becomes a Hanging Drop
  Researchers apply acoustic liquid-transfer technology to protein crystallization.
  
• New Luciferins
  Researchers describe a rapid method to synthesize D-luciferin along with a novel set of electronically modified analogues.
  
• Reducing Endocytosis Assay
  Researchers describe a new method of studying the endocytosis and recycling of cell surface proteins.
  
• Biographies for Compounds
  Researchers demonstrate the value of a tool that compares compounds solely on the basis of their bioactivity for virtual screening.
  
• ATP Gets Smart/Absorbing BLA Assay
  Researchers propose a fluorescent probe for adenosine triphosphate assay and describe a synthesis of a chromogenic cephalosporin for β-lactamase assay.
  
• Binding MoA by DPI
  Researchers investigate the application of dual-polarization interferometry (DPI) as a method for the real-time characterization of low molecular weight ligands.
  
• Tripping The Light Fantastic: New Reporter System
  This study outlines an approach to improved use of reporter genes in high-throughput screening.
  
• Look, No Label! Label-Free MST
  Two articles report on two methods that provided different levels of information on the differentiation of binding sites available on protein targets of interest.
  
• Literature Review: Mega-Selection
  Researchers present a method that uses combinatorial antibody libraries to endow cells with new binding energy landscapes to regulate their phenotypes.
  
• Literature Review: ELISA—Detected Through A Quick Glance
  Researchers provide an assay chemistry that allows for a direct visual scoring of an ELISA result to detect protein analytes at ultralow concentrations.
  
• Literature Review: Protein—Now You See It, Now You Don't
  Researchers describe a system for the rapid and complete degradation of proteins in mammalian cells.
  
• Literature Review: Bacteria Reprogram Host Cells in Order to Survive and Spread
  Researchers describe how leprosy bacilli can change Schwann cells to stem cell-like cells to promote infection.
  
• Literature Review: Which Drug Combination Will Unlock Triple-Negative Breast Cancer?
  Researchers describe a strategy for treating TNBC that combines noncompetitive antibodies to achieve degradation of EGFR and tumor inhibition.
  
• Literature Review: A Step Closer to Personalized Medicine
  Researchers use patient-specific cell lines derived in mice to personalize chemotherapy.