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Insight & Intelligence™ : Aug 20, 2014

The In Vivo and In Vitro Transfection Marketplace

Learn how cells and animal models each factor in this space.
  • Enal Razvi, Ph.D.

The focus of this GEN Market & Tech analysis report is to discuss some of our data on in vitro transfection into cells in culture as well as present some in vivo transfection data (transfection into animal model systems). This report is an extension of a report on transfection that we published several months ago; the focus of that report was on in vitro transfection into cells primarily.

Highlights:

  • We present the market landscape for transfection in this report—an important front-end approach underlying the analysis of genetic constructs such as cDNAs in a biologically relevant context—i.e., in the cellular milieu.
  • Lipid-based transfection into cells is the most penetrant approach currently in the marketplace.
  • Tissue cultured cells are much more prevalent as targets for transfection as opposed to explanted primary cells.
  • The most important factors underlying a transfection technology are: High Transfection Efficiency, Low Cytotoxicity to Cells, and Reproducibility (across experiment).
  • cDNA constructs are the most penetrant genetic payload in transfection experiments currently.