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Feature Articles : Feb 15, 2009 ( )
Case Study: Overcoming Antimicrobial Resistance
Pitting Topical, Nonantibiotic Agents against Systemic Antibiotic Overkill!--h2>
Antibiotics may rightly be called wonder drugs, but their use over the last 80 years has come at a price. Through evolutionary mechanisms assisted by overuse and misuse, bacteria develop resistance to new antibiotic compounds soon after their introduction. Today, some of the most virulent bacterial pathogens are resistant to all but one or two agents.
Our experience with one topical antibiotic, mupirocin, demonstrates that resistance can emerge even against agents that are not administered systemically.
GlaxoSmithKline’s Bactroban (mupirocin ointment) anti-infective was introduced in 1985 and rapidly adopted into clinical practice for treating topical Staphylococcus infections and colonizations. Numerous studies demonstrated mupirocin’s effectiveness in treating primary skin infections, surgical incisions, and accidental wounds. Bactroban soon became the agent of choice for these indications; within 15 years the drug was registered in 90 countries for eradication of Staphylococcus, including such virulent strains as methicillin-resistant S. aureus (MRSA).
Resistance to mupirocin began to emerge shortly after the drug’s introduction. By 2007 Simor et al. reported that the incidence of mupirocin-resistant Staphylococcus aureus increased from 1.6% during the period 1995–1999, to 7% between 2000 and 2004. Resistance was related to a mutation on a gene coding for the enzyme isoleucyl-tRNA synthetase. Moreover, it became apparent that MRSA could confer resistance to mupirocin through gene transfer to other bacteria treatment.
A more recent study, by Fawley et al, on perioperative patients, confirmed that 7% of Staphylococcus isolates from nasal passages of orthopedic/vascular patients were mupirocin-resistant, a figure that rises to 9% among elderly patients. In 2007, David Warren and coworkers reported that 13.2% of MRSA isolates from patients at Washington University hospital were mupirocin-resistant. These figures have immediate consequences, as Graber et al. noted failures in decolonization in patients infected with mupirocin-resistant MRSA.
Bacterial resistance to antibiotics generally rose throughout the 1990s and 2000s, and will continue to increase despite efforts to introduce “clean” treatment practices in hospitals. The availability of over-the-counter antimicrobial agents, particularly those that were once sold only by prescription, can potentially reverse the positive impact of best hospital practices, and lead to pockets of high bacterial resistance that will be difficult to eradicate.
For example, a study by Upton and coworkers reported that mupirocin resistance in New Zealand hospitals had reached 28% by 1999, due in part to sales of mupirocin over the counter. Upton urged that “current patterns of mupirocin consumption be reviewed and its use rationalized to maximize the chances of this antibiotic retaining beneficial antistaphylococcal activity.”
Mupirocin is a good antibiotic, but therein lies the problem. Bacteria have evolved over hundreds of millions of years to evade and adapt to antibiotic mechanisms, particularly when these agents are administered systemically. It, therefore, makes no sense to expose every organ and system to antibiotic treatment when an infection is localized to one area that is easily accessible to topical agents. An unintended consequence of the overuse of systemic antibiotics has been the rise of resistant strains on the skin, which complicates treatment even in accessible areas of the body.
Antibiotic Discovery Conundrum
Since 1941, when penicillin was introduced in the United States, every antibiotic brought to market has become less effective (or in some cases completely ineffective) thanks to bacterial resistance. Beginning in the early 1990s this unavoidable problem, coupled with low profit margins, made antibiotics an unattractive business proposition. Companies saw little benefit in developing yet another variant of beta-lactam, quinoline, or macrolide antibacterials as these agents were, for want of a better term, “played out.”
That is beginning to change. The sequencing of the first bacterial genome in 1995 presented the opportunity to create new classes of antibiotics that operated through novel mechanisms against both old and new targets. Realizing the full potential of genome mining is still several years off. Thus far, results have been mixed because antibiotic drug discovery, even when based on gene mining, results in only one-fifth of the number of lead compounds per screening program as for other therapeutic areas.
Nevertheless, the rise in resistant super-bugs has created renewed interest in antibiotics. But even those that operate through novel mechanisms share the fatal flaw of first-line agents, namely that bacteria will eventually adapt, and the agents will become less effective.
The intravenous agent Ceftobiprole (zeftera; Johnson & Johnson), currently under FDA review, is a case in point. Ceftobiprole has been hailed as a fifth-generation antibiotic, partly because it short-circuits known resistance mechanisms. For example, Ceftobiprole is active against MRSA and resistant to staphylococcal beta-lactamase, the key mutation that confers resistance to beta-lactam antibiotics. Despite the high billing, researchers have recently discovered Ceftobiprole-resistant strains of S. aureus by simple passaging experiments.
Natural anti-infective molecules such as antimicrobial peptides would seem like a promising starting point for new antibiotic development. Several groups have demonstrated the potential of defensins, host defense peptides. Unfortunately, defensins are generated by neutrophils, which along with a narrow chemical and nutrient environment are required for activity.
While they are often promising in vitro and in animal models, peptides have numerous problems as drugs. They are difficult to manufacture on a large scale. Degradation in the digestive tract makes peptides unsuitable for oral delivery, and the molecules generally have a short physiologic half-life. As several groups have pointed out, defensins are simply not druggable.
Nonmammalian organisms are a potentially fruitful source of novel, natural antibiotic compounds. The rationale for this approach is that organisms that are immune to S. aureus probably possess a fail-safe chemical-defense mechanism against infection.
For example, a group at Philadelphia’s Wistar Institute have discovered an insect-derived class of antimicrobial agent, known as pyrrhocoricins, which are active against a range of resistant bacteria. Laszlo Otvos, lead researcher, created chemical analogs of pyrrhocoricin to isolate bactericidal and cell-entry characteristics of the original molecule. Otvos’ approach is quite promising because it is based, not on a cellular mechanism, but on binding to an essential bacterial molecule. Pyrrhocoricins bind to DnaK, a heat-shock protein used to repair faulty bacterial proteins. Inhibiting DnaK causes protein mistakes to build up, eventually killing the bacterium.
The litmus test for pyrrhocoricin and defensin analogs, and other discovery-stage agents, will be their manufacturability, pharmacokinetics, clinical efficacy, and ultimately whether they promote resistance from the infectious agents they are supposed to fight.
Antimicrobials, Not Antibiotics
In a recent paper, researchers at NovaBay described a novel class of antimicrobial compounds known as N,N-dichloro-2,2-dimethyltaurines (Aganocides®), which are effective against MRSA and mupirocin-resistant Staphylococcus. Aganocides belong to a class of naturally occurring antimicrobial agents, the N-chlorotaurines, that operate within the human immune system and do not give rise to bacterial resistance of any kind.
The natural model for Aganocides, N-chlorotaurine, was described in 2000 as a novel agent for treating infectious conjunctivitis. A number of papers have been published using this compound as a topical antimicrobial agent. Nagl et al. reported the broad-spectrum biological activity of the long-lived oxidant N-chlorotaurine, which achieves 4-log reduction of bacterial and fungal pathogens at micromolar concentrations.
Biologists know that species related to N-chlorotaurine are responsible for up to 90% of the heavy lifting in bacterial clearance through white blood cell lysosomes. During oxidative bursts, hypochlorous acid is neutralized by taurine to form N-chlorotaurine, an oxidant that attacks and inactivates bacteria and other pathogens. N-chlorotaurine and the related N-dichlorotaurine possess broad-spectrum antimicrobial activity, but both degrade rapidly in the body and are labile in conventional pharmaceutical formulations. Aganocide compounds overcome this deficiency of the natural compounds through a chemical modification that renders them more stable.
Like their natural analogs, Aganocide compounds fight MRSA and other resistant Staphylococcus bacteria through the chloronium ion, a form of chlorine suitable for eradicating bacterial colonizations and infections on the skin, in other accessible areas of the body, and on some implantable medical devices. The Chloronium ion has been employed in water disinfection for at least 150 years.
Aganocides, which deliver an attenuated form of chloronium ion, are not antibiotics. Their mode of action is nonspecific and does not depend on cells being in reproductive phase. Aganocides do not inhibit cellular processes, DNA replication, enzymes, or any pathways that might, through evolutionary processes, adapt to their mode of action.
Rather, chloronium ions generated by Aganocides rapidly inactivate organisms by attacking sulfur- and nitrogen-containing amino acids on the bacterium’s surface. Microorganisms cannot adapt, either individually or through evolutionary processes, to this mode of action, which is not unlike being run over by a Sherman tank. Developing immunity to Aganocide compounds would require that MRSA bacteria completely change their chemical composition.
In this respect, Aganocides resemble antimicrobial peptides, another group of natural defense compounds that do not induce microbial resistance. Antimicrobial peptides are evolutionarily conserved, meaning their structures are similar across species and over millions of years of evolution. These agents are usually amphiphilic, allowing them to operate in aqueous environments yet also enter lipid-rich membranes. Unfortunately, no antimicrobial peptide or analog has proved to be commercially viable, since their selectivity for bacterial vs. mammalian membranes is too low.
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