GEN Exclusives

More »

GEN News Highlights

More »
Jan 6, 2011

Researchers Develop Simpler, Quicker Way of Producing AAV-Based Vectors for Gene Transfer

  • Scientists at the Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, have developed a method for producing large amounts of viral vector cassettes to shuttle genes into host cells. The researchers report that their simplified method offers important advantages including the potential to produce high yields of multiple adeno-associated vector (AAV) serotypes upon co-infection with a helper adenovirus as well as efficient packaging of single- or double-stranded AAV vectors and of large AAV cassettes.

    Their findings are described in a paper titled, “A versatile AAV producer cell line method for scalable vector production of different serotypes,” which is available online ahead of publication in Human Gene Therapy.

    The new method for creating stable AAV2 producer cell lines reportedly overcomes major drawbacks seen in previously used methods. Rather than relying on multiple cloning steps, the new method reduces it to a single step. In addition, a formerly two-stage transfection and selection step has been reduced to one step while still producing cell lines containing the circular, AAV vector-containing plasmid needed to transport a gene of interest into host cells.

Add a comment

  • You must be signed in to perform this action.
    Click here to Login or Register for free.
    You will be taken back to your selected item after Login/Registration.

Related content


GEN Jobs powered by connects you directly to employers in pharma, biotech, and the life sciences. View 40 to 50 fresh job postings daily or search for employment opportunities including those in R&D, clinical research, QA/QC, biomanufacturing, and regulatory affairs.
More »

Be sure to take the GEN Poll

Easing Restrictions for Terminal Patients

Should the Federal Government Pass a “Right to Try” Bill Allowing Terminally Ill Patients Access to Experimental Medicines?

More »