Leading the Way in Life Science Technologies

GEN Exclusives

More »

GEN News Highlights

More »
Apr 20, 2010

Ghent University to Leverage Roche´s LightCycler® 1536 Instrument for Cancer Research

  • Ghent University has decided to use Roche's LightCycler® 1536 Instrument in various cancer research projects. Studies include multigene expression signature profiling on cancer research samples and digital PCR for detection of mutant cancer cells in a background of normal cells.

    Furthermore, DNA methylation analysis studies, copy number variant screening, and amplicon generation for next-generation sequencing are part of the planned experiments. Ghent researchers also point to one study in particular, which is a qPCR-based transcriptome-wide profiling. In a pilot experiment, Ghent University plans to measure all human genes in the four MAQC reference RNA samples.

    The LightCycler® 1536 Instrument supports the combination of two excitation filters with two detection filters, which are optimized for detecting green intercalating dyes as well as monocolor and dual-color hydrolysis probes. This makes optical read-out as specific as possible for chemical detection formats, while reducing the overall complexity of experimental layouts in a high-throughput scenario, according to Roche.

    The LightCycler® 1536 Multiwell Plate is a high-density PCR plate for real-time PCR applications. By using Thermaxis® technology, this multiwell plate enables thermal performance in miniaturized reaction volumes of 0.5–2 µL. The plate consists of two components: a thermally conductive unit containing well-like structures for the reaction liquid and an insulating top layer that prevents the heated lid of the instrument from affecting the analysis.



Related content

Be sure to take the GEN Poll

Cancer vs. Zika: What Worries You Most?

While Zika continues to garner a lot of news coverage, a Mayo Clinic survey reveals that Americans believe the country’s most significant healthcare challenge is cancer. Compared to other diseases, does the possibility of developing cancer worry you the most?

More »