Multiplexing to Improve Optimization
Tracking cell viability along with reporter activity is critical to determining optimal transfection conditions. High reporter activity may come at the expense of cell health, and unhealthy cells are less likely to exhibit consistent, physiologically relevant responses.
Optimization is simplified further by using reporter and viability assays that can be measured in the same sample. The ONE-Glo™ Luciferase Assay System and the CellTiter-Fluor™ Cell Viability Assay are compatible assays. By multiplexing these two assays, both reporter activity and viability can be measured in the same well of a 96-well plate in less than an hour. No medium changes or washing are needed.