Microvolume Quality Control
The implementation of microvolume UV testing at-line eliminates batch testing, greatly reducing processing time, and increasing efficiency. Microvolume UV analysis is performed using Thermo Scientific NanoDrop™ 8000 spectrophotometers. Each spectrophotometer measures multiple protein samples using a sample-retention system that requires small amounts (~2 µL) of protein sample. The system uses the inherent physical properties, namely surface tension, to hold samples in place during measurement, eliminating the need for cuvettes, capillaries, or other containment devices.
Optical pedestals hold and measure up to eight microvolume protein samples at a time. The removal of classic containment devices allows the path length between the optical pedestals to change during the measurement cycle. The ability to optimize path length results in an extensive dynamic range of possible protein concentrations that can be measured (e.g., 0.1 mg/mL to 120 mg/mL for HSA), essentially eliminating the need to perform time-consuming dilutions and the errors associated with preparing such dilutions (Figure 1).