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May 15, 2010 (Vol. 30, No. 10)

Upping the Sensitivity and Accuracy of qPCR

Double-Quenched Probes Designed to Be Stable at High Temperatures and Minimize Fluorescence

  • Conclusion

    As a sensitive technique with many uses, it is vital that data obtained from qPCR is as accurate and reliable as possible. IDT’s new double-quenched probe, with the internal ZEN quencher, enables researchers to obtain highly sensitive results due to the significant reduction in background fluorescence.

    Furthermore, providing a solution in which the quencher remains close to the fluorophore, regardless of probe length, enables the use of longer probes with a higher Tm, which are able to remain stable at higher temperatures. Researchers can, therefore, be confident in the data obtained from their qPCR.

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