Conclusion
As a sensitive technique with many uses, it is vital that data obtained from qPCR is as accurate and reliable as possible. IDT’s new double-quenched probe, with the internal ZEN quencher, enables researchers to obtain highly sensitive results due to the significant reduction in background fluorescence.
Furthermore, providing a solution in which the quencher remains close to the fluorophore, regardless of probe length, enables the use of longer probes with a higher Tm, which are able to remain stable at higher temperatures. Researchers can, therefore, be confident in the data obtained from their qPCR.