Promega offers a number of cell-based assays based on novel bioluminescent and fluorescent chemistries that can be combined in the same well, providing more biologically relevant information per well of cells. Moreover, the newer assay chemistries are easy to implement on robotics platforms and scalable up to 1,536-well plates, according to Pam Guthmiller, strategic marketing manager for cellular analysis.
“By developing assay chemistries that can be multiplexed in the same well, the researcher can now perform these assays in microwell plates using a simple multimode detection instrument. Because of the simplicity and sensitivity, a number of life science researchers are turning to these formats.”
The offerings are versatile and sensitive, according to Guthmiller, and include the GloMax®-Multi detection plate reader system for measuring a broad range of fluorescent and luminescent cell signals. Many of the assays are streamlined through a homogeneous add-mix-measure format, meaning that removing or changing the medium is unnecessary. The various substrates are simply added to the culture medium as a concentrate and then read. Examples include the MultiTox-Fluor multiplex cytotoxicity assay (measures viability and cytotoxicity) combined with the Caspase-Glo® 3/7 assay, designed to provide data on the role of apoptosis in cell cytotoxicity.
These assays can also be combined with reporter assays to gain information on gene expression when cells are exposed to potential therapeutic drugs. The ONE-Glo™ luciferase reporter assay system can measure transcription and translation. Combined with the CellTiter-Fluor™ cell-viability assay, evidence can be assembled on the concordance between gene expression analysis, cell viability, and cytotoxicity within a single assay.
Another key product is the nonlytic, luminescent CytoTox-Glo™ cytotoxicity assay. “It can detect small changes in viable cell number and also can be multiplexed with fluorescent-based assays, improving data quality and saving time, money, and reagents,” says Guthmiller.