Single-use bioprocessing product lines made their debut mainly in the form of disposable bags for mixing and buffer supply. They gradually moved into the heart of the bioprocess stream with the emergence of single-use bioreactor systems.
Single use has more recently made inroads in downstream process flows for separation, purification, and fill and finish applications. This evolution in the industry is clearly illustrated at bioprocessing conferences, in which presentations highlight the range of product areas and biopharmaceutical R&D and manufacturing applications targeted by single-use technologies.
Examples include IBC’s recent “Single-Use Applications for Biopharmaceutical Manufacturing” conference in San Francisco, and Visongain’s upcoming “Single Use Bioreactor” conference in London. Compared to early single-use bioreactors, which were essentially disposable bags mounted on platforms to achieve mixing, the emerging, more complex generation of single-use systems more closely mimic conventional stirred-tank glass and stainless steel bioreactors. They incorporate impellers, sparge lines, and sensors for better control of process parameters and have aspect ratios that mirror those of traditional stirred tank reactors.
With the launch of its new ambr 250™ mL automated, scalable, single-use bioreactor system in August, TAP Biosystems aimed to increase the complexity and functionality of single-use bioreactors for use in process development while maintaining their ease of use in terms of set-up, operation, and user interface.
Andrew Tait, product development scientist at TAP Biosystems, will describe the company’s new technology and how it achieves these dual goals in his presentation at the Visongain conference.
Meeting its initial goal meant providing core stirred tank bioreactor technology for both microbial and mammalian applications, with an impeller and width-to-height ratios of conventional steel bioreactors. The system’s single-use bioreactor incorporates gas and liquid supply lines with connectivity to the bioreactor control unit.
The system is configurable for automated, independent, parallel control of 12 or 24 bioreactors, each with a maximum working volume of 250 mL. Each bioreactor can be individually controlled for temperature, pH, and dissolved oxygen, has four liquid feed lines, and off-gas analyzers for microbial applications. Up to 24 bioreactors are integrated into a dedicated biosafety cabinet.