The iuvo platform is designed to interface with existing lab automation, including liquid handlers and high-content analysis instruments. The spacing between input ports is 9 mm horizontally and 4.5 mm vertically. This allows a 96-channel head to dispense to all 192 channels in two steps. The entire assay, including cell addition, media changes, and post-assay staining is performed by adding or removing 2–5 µL droplets from the ports. No manual interventions of any kind are required.
Fluid flow in the channel is driven by droplet-based passive pumping, which relies on the pressure differentials inside droplets of different radii at air-liquid interfaces. For detection of cell positions, cells are stained with fluorescent nuclear dyes, imaged on automated microscopes or high-content analysis instruments, and analyzed using standard object identification algorithms.