Implementation of process-ready hardware systems containing single-use processing assemblies permits rapid facility start-up as well as ongoing operational flexibility. This study presents data demonstrating that a preengineered system for TFF can deliver optimal process performance in terms of yield, quality, and product purity through implementation of low working volume, excellent product recovery, effective mixing, and efficient removal of small molecules.
The Mobius FlexReady TF-2 system enabled greater than 98% product recovery from even the most concentrated model protein feedstock tested, while minimizing turbulence during mixing and recirculation to maintain high product quality. Greater than 4 logs clearance of small molecules during buffer exchange delivers performance that is equivalent to traditional stainless steel TFF systems.
The data demonstrates that pre-engineered systems combining process-ready hardware systems with single-use, application-specific fluid flowpath assemblies are an effective means of realizing the full benefits of single-use technology.