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April 01, 2006 (Vol. 26, No. 7)

Technical Note: Highly Sensitive qRT-PCR from One Cell

RNA Amplification & cDNA Synthesis Enables Sensitive qRT-PCR of Low-Abundance Transcripts

  • Ideally, gene expression studies by quantitative RT-PCR (qRT-PCR) should be performed using RNA from homogeneous cell populations. Frequently, this means performing qRT-PCR using picogram amounts of total cellular RNA and, in some instances, using total RNA from a single cell. Technologies such as laser capture microdissection and fluorescence-activated ...

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