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Oct 15, 2011 (Vol. 31, No. 18)

Target-Enrichment Field Continuing to Expand

Cost Advantages Over Whole-Genome Sequencing Likely Main Factor in Growth

  • Click Image To Enlarge +
    An effective target-enrichment strategy allows scientists to focus on significant regions of genomic interest before beginning sequencing activities. By working on statistically relevant samples, the researchers’ overall laboratory efficiency will increase. [Martin Shields/Photo Researchers]

    As a result of recent progress in DNA sequencing technologies, which has generated datasets of unprecedented complexity and advanced our understanding of health, disease, and development, whole-genome sequencing has emerged as a highly anticipated goal. However, routine sequencing of whole genomes is still a challenging milestone, particularly for clinical applications.

    A more affordable approach, target enrichment, involves capturing genomic regions of interest prior to sequencing. One example is whole-exome sequencing, in which next-generation technologies sequence only the coding regions, which represent slightly over 1% of the human genome and are thought to harbor a large proportion of the variation associated with human disease.

    “A few years ago, I would have thought that targeted sequencing would be influential for only a few years, and that it would be replaced, as sequencing costs come down, by whole-genome sequencing, but that has not happened yet,” says Chad Nusbaum, Ph.D., co-director of the genome sequencing and analysis program at the Broad Institute.

    Dr. Nusbaum and colleagues developed an approach called Solution Hybrid Selection capture, in which biotinylated RNA capture bait probes are generated and mixed with a library of randomly sheared DNA fragments amplified from human genomic DNA that are modified with sequencing adaptors. Hybridized fragments are captured on streptavidin beads, and the DNA is sequenced on a next-generation platform.

    This method facilitated the extensive sequencing of targeted genomic loci of interest. After optimizing and further developing this application, investigators from Dr. Nusbaum’s lab recently described the first automated, highly scalable application to perform Solution Hybrid Selection capture in a cost-effective and highly efficient manner.

    Several target-enrichment approaches have emerged in recent years. A factor that has catalyzed the expansion of this field is that as long as the costs remain significantly lower than the costs of sequencing whole genomes, more samples can be analyzed. “Since human genetics is all about statistics, and statistics is driven by the number of samples, targeted sequencing remains a significantly growing area,” says Dr. Nusbaum.

    Among target-enrichment platforms, exome sequencing has received increasing attention. To a great extent, this can be explained by our better understanding of the direct biological implications of sequence variations within exons. “The remaining 97–98 percent of the genome, while it cannot be ignored and we need to learn more about it, is still difficult to understand in terms of biological and clinical implications,” emphasizes Dr. Nusbaum.

    “We were interested in examining quantitative trait loci, and after localizing a specific genomic region identified by linkage analysis, we adopted the emerging technology of targeted resequencing,” says Jeremy B.M. Jowett, Ph.D., head of genomics and systems biology at the Baker IDI Heart and Diabetes Institute from Melbourne.

  • Click Image To Enlarge +
    Agilent Technologies’ SureSelect Target Enrichment kits are available to capture target sizes ranging from smaller than 200 Kb to more than 50 Mb in a single tube. The in-solution format is automation-friendly, and is compatible with all major next-generation sequencing platforms.

    By using the Agilent Technologies SureSelect target-enrichment system, Dr. Jowett and colleagues reported the possibility to combine index barcode multiplexing with solution-based target enrichment. The authors genotyped a 3.3 Mb region on the X chromosome in five individuals, and illustrated the strength of this approach in detecting most SNPs within the region, with a concomitant decrease in the time and costs involved.

    “As this application becomes more mature and more robust, it will also find its way into the clinic,” says Dr. Jowett.

    The ultimate goal, particularly when looking for mutations associated with complex diseases, is to perform whole-genome sequencing to capture all the genetic variation, quantitate the effect on disease risk, and identify regions and variants associated with specific conditions. Nevertheless, at least at present, this approach is challenging. “It might be too expensive to sequence whole genomes in hundreds of people, but it is possible, and within the budget of many labs, to conduct exome sequencing,” says Dr. Jowett.

    The choice between whole-genome sequencing and target-enrichment depends on the specific application. Whole-genome sequencing could be the method of choice for research applications, but in the clinic, it might not be the preferred option, as it is more efficient to identify and focus on the genomic regions that are important for specific conditions and subsequently perform target enrichment.

    “It may also be possible that the paradigm will be to initially focus on specific genomic regions containing genes associated with a disease, and this could involve a dozen or so different genomic regions, and if this would not reveal anything, there may be an additional step toward whole-genome sequencing.”

  • Exome Sequences

    In many research articles, investigators perform exome sequencing to identify genes that are mutated in specific medical conditions. “We pursued a different approach, and in a cohort of people who had their genomes sequenced for completely unrelated reasons, we wanted to find out how many individuals have variations in a specific recessive disease gene,” says Leslie G. Biesecker, M.D., chief and senior investigator at the genetic disease research branch at the NIH.

    Dr. Biesecker and colleagues relied on the ClinSeq cohort, a pilot project that uses whole-genome sequencing to investigate the genetic basis of health, disease, and drug response, and currently enrolls close to 1,000 individuals, in which the whole exome sequences are available for approximately 600.

    The authors looked for genes involved in combined malonic and methylmalonic aciduria, a recessive Mendelian disorder, and found mutations in ACSF3, marking the first time a human disorder was causally linked to variations in an acyl-CoA synthase family member.

    “We examined the exome sequences to find out how many individuals in the cohort have variations in that gene,” explains Dr. Biesecker. This approach not only identified recessive carriers of the mutation but also unveiled an example when two mutations were present in the gene.

    “We previously thought that this is a severe disease with childhood onset, and finding in this adult cohort a patient with two mutations in the gene was quite unexpected.” Additional metabolic testing on the patient confirmed the disease, and revealed that this disease is not only a childhood onset severe metabolic disorder, but may also present as an adult onset mild condition that masquerades as a neurodegenerative disease later on life.

    “This tells us that a genome-based approach may identify individuals with phenotypes that we do not even know that we should be looking for, and we can identify them that way.”

    For certain biological questions, sequencing several exomes is more powerful than generating one whole-genome sequence, and this concept is illustrated by another recent finding from Dr. Biesecker’s lab. Dr. Biesecker and colleagues recently examined patients with the Proteus syndrome, a rare developmental condition with multisystem involvement and broad clinical variability, characterized by severe malformations and overgrowth of multiple tissues.

    Exome sequencing from affected and unaffected tissues unveiled somatic mutations in AKT1 in 26 of 29 individuals with this condition, only in the affected tissues. “We also conducted high coverage whole-genome shotgun sequencing in a patient with this condition, and on a paired set of unaffected and affected tissues, we did not see the alteration,” reveals Dr. Biesecker.

    “This is a clear example when sometimes lower costs of an exome, and the ability to interrogate more samples is more effective, whereas the whole genome, even though it provides more coverage, could miss things.”

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