CC(3) Labware vs. Poly-d-lysine
The growth of three transformed cell lines (CHO, HEK-293, and BHK) on the CC3 surface was compared to a PDL-coated surface in both normal and reduced serum media. Because neurons often prefer PDL surfaces, the growth of a neuron-like cell line (PC12) was also compared on both PDL and CC3 (with serum only). Stability of the CC3 surface was determined via an accelerated aging study.
All cell lines used in this study were obtained from the American Type Culture Collection (ATCC). Examples of applications for each cell line include the study of cell differentiation and cancer research (PC12), bioproduction of proteins (HEK293), antibody production (CHO), and vaccine production (BHK).
Ninety CC3 plates sterilized to an SAL of 10-6 were produced. The cell lines were seeded onto both the CC3 and poly-d-lysine coated plates in media with serum as recommended by ATCC: HEK293 and CHO, 10% fetal bovine serum medium; BHK, 10% bovine calf serum medium; and, PC12, 15% horse serum medium and 2.5% fetal bovine serum media.
To compare CC3 and poly-d-lysine in reduced serum media, HEK, CHO, and BHK cells were gradually adapted from 10% serum media into reduced serum media (5%, 2%, and 1%) over the course of four passages. The three cell lines were then cultured onto both CC3 and poly-d-lysine coated plates. All plates were then incubated at 37C.
To test stability, CC3 plates were aged in an accelerated program. A total of sixty CC3 plates were aged either two or five years using an aging method in accordance with AAMI/ISO guidelines. Aged plates were compared to one-month old CC3 plates with HEK cells in 10% fetal bovine serum medium.
For analysis, the cells were fixed in 4% formaldehyde and stained in crystal violet to compare the level of cell attachment and growth.