Alvetex satisfies all these criteria and is available in the base of multiwell plates and a custom well insert (Figure 2). Like plasticware for conventional 2-D culture, alvetex comes sterilized, prepacked, and ready to use. It is a cost-effective solution for routine 3-D cell culture, which is readily transferred between laboratories and its use does not require the purchase of any specialized equipment.
There are significant differences between how 2-D and 3-D cell culture is practiced and such differences often follow common logic. For example, in 3-D culture there tend to be many more cells per unit volume of medium compared to the equivalent 2-D system. Therefore, the frequency of media changes needs to be increased. However, this might not be the preferred or most convenient option.