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March 01, 2007 (Vol. 27, No. 5)

Supplement Article: RT-PCR Enters the Realm of Stochastic Gene Expression

  • With the introduction of fluorogenic probes and fluorometric thermal cyclers that enable the monitoring of amplification reactions in real time, the sensitivity and precision of gene expression analyses has improved dramatically. Utilizing these assays, often called quantitative or real-time RT-PCR, populations of mRNAs can now be analyzed from samples ...

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