Crosslinked protein complexes can be detected by reduced SDS-PAGE mobility followed by Western blotting.
In Vivo Crosslinking of Live Cells
To demonstrate photoreactive crosslinking in live cells, HeLa cells were treated with Photo-Leucine and Photo-Methionine before photoactivation with UV light. For comparison, HeLa cells were also crosslinked using formaldehyde, DSG, and DSS. Treated cells were then lysed and analyzed by SDS-PAGE and Western blotting using an antibody against the homodimeric transcription factor Stat3.