ligned with customer requirements and the trend toward QbD, Kerry developed cell-line specific complex media supplements. Proprietary process technology and innovative media optimization methods have been applied to develop specific, homogeneous, functional supplements from combinations of complex and chemically defined animal-component free media additives.
As an example, the Sheff-CHO system is a series of complete medium supplements optimized for CHO cells. The systems contain mixtures of factors known to promote CHO cell growth, cell metabolism, and protein production and have been carefully optimized to give superior recombinant protein yields.
The advantage of applying cell line specific supplements such as Sheff-CHO Plus ACF and Sheff-Pulse both in batch and fed batch cultures was recently investigated. Cells were seeded at 4.0 x 105 cells/mL and grown in 125 mL shake flask to a final volume of 50 mL CDM or DMEM plus Sheff-CHO Plus ACF at 9 g/L for batch cultures. For fed batch cultures media was supplemented at day 5, 7, 9, and 12 with basal media or a proprietary blend of Sheff-CHO Plus ACF and Sheff-Pulse. 1 mL samples were taken at day 0, 5, 7, 8, 9 to assess culture viability using a Nova BioProfile Flex automated analyzer. At day 12 product titer was analyzed via HPLC.
A similar study was set up to compare the Sheff-Pulse system with competitor feeds. Experimental design was as described above with cultures fed at day 5, 7, 9, and 12 and samples taken throughout the culture process to assess cell performance with respect to growth and productivity.