Solutions for Complex Applications
Genetix has focused on developing families of instrumentation and complementary consumables for the markets increasingly complex colony picking/arraying applications. The original QPix platform has been joined by the QPix2XT, which features automated microwell plate delivery and tracking, and its application-based derivatives, including the QPExpression, that is capable of automating bacterial or yeast cloning methods requiring plating out, spreading, and colony picking; the QPEvolution, designed to automate the colony selection, picking, and hit-selection steps of protein engineering methods; and the recently launched QPDisplay, designed as a dedicated picking and library-management system for applications in phage display.
The need for mammalian cell-based screening and colony picking systems is evidenced by the success of the ClonePix, launched in January 2004 and the ClonePixFL, launched in January 2005.
The ClonePix uses white light to identify and pick individual mammalian cell colonies, including hybridomas in semisolid media, and adherent cells, including CHO and HEK, Genetix maintains. The more recent ClonePixFL platform is designed for imaging, identification, picking, and stratification of mammalian colonies based either on quantified secretion of unlabeled proteins or expression of fluorescence-labeled transfected proteins.
Featuring automated delivery and de-lidding of 6-well culture plates to the on-board imaging station, the system uses fluorescence imaging with a choice of filter sets to rank and select colonies based on parameters, including size, brightness, proximity to neighbors, and, where appropriate, halo-size. Picked colonies are then dispersed in 96-well plates.