Bioanalytical Systems (www.bioanalytical.com) offers the Sample Sentinel Programmable Autosampler, which provides a robotic approach to liquid chromatography autosampling for microbore, standard bore, or preparative applications. By moving the sample vial to the sampling, diluting, or mixing stations, the autosampler eliminates the need for complex mechanical drivers, according to the company.
A sample cooling option is available for applications involving labile samples requiring storage at reduced temperatures, and a sample preparation capability option enables the unit to provide multiple derivatization and dilution functions, as well as vortex mixing with selectable heating of the vortex chamber.
WellMate from Matrix Technologies (www.matrixtechcorp.com) offers disposable, presterilized tubing cartridges and dispensing in a wide volume range (12,000 L). It accommodates microplates from 6 to 384 wells or any height 96-position tube rack.
For automatic filling of multiple microplates, Matrix offers the WellMate stacker, an automatic plate storage and handling module. The stacker unit shuttles microplates between the stacker unit and the WellMate dispensing head for unattended microplate filling for up to 25 or 50 microplates.
By raising or lowering the dispensing head to the required height, WellMate can handle anything from deep and shallow well plates to storage tubes as tall as 89 mm. Disposable, presterilized tubing cartridges have standard or small-bore needle options.
GenPlates are the core of GenVault's (www.genvault.com) integrated biosample management system, according to David Wellis, vp, business development. This building block eliminates the need for freezers and liquid nitrogen tanks while maintaining biosamples in a stable format suitable for long term archiving, automated retrieval, and simplified distribution, he says.
The 384-well GenPlate contains 6-mm disc-shaped elements of Whatman (www.whatman.com) FTA paper in each well and allows biosamples to be stored at room temperature.
Each element within the GenPlate is an experiment-sized aliquot that can be removed without risk of cross-contamination or sample degradation from multiple freeze-thaw cycles. Additionally, the GenPlate is approximately half the height of a standard multiwell plate for maximum storage efficiency.
The basic GenPlate, which is configured to hold a single patient sample across 384 aliquots, requires about 4 mL of biological fluid to fill the entire plate. The sample is applied directly to each element within the GenPlate and allowed to dry.
Cells within the sample lyse upon contact with the FTA material and the extruded DNA binds to the cellulose matrix. Then the GenPlate is sealed and ready for short- or long-term archiving.
FTA inactivates bacteria and viruses upon contact, allowing sample shipping without any biohazard labeling. DNA stored on FTA paper as long as 14 years ago has been recovered in sufficient quantity and quality for current genotyping techniques, Wellis adds.
Once a sample is applied to a GenPlate, chain of custody is established. Each GenPlate is labeled with a unique physical barcode, and each element within the GenPlate is labeled with GenCode, GenVault's biological barcode, which permanently identifies the actual sample regardless of its physical state.
GenVault and Whatman are collaborating on the EasyClone 384 plate, which is designed to deliver shipping, storage, and backup of sample or samples to the clone market by formatting Whatman technology into a 384-well storage and extraction plate designed by GenVault. The plates will be based on the FTA technology.