TGX gels retain superior Laemmli-like separation characteristics using standard sample and Tris-glycine running buffers. The patented modification of the Laemmli buffer system has reduced hydrolysis of the gel matrix over time, thereby extending shelf life up to 12 months and delivering consistent, high-quality performance.
At 300 V, a typical run can be completed in 15 minutes (compared to 30–45 minutes for other precast and handcast gels). This is because TGX gels can be run at higher voltages than other gels without impacting performance (Figure 1). The subsequent transfer step can be completed in 15 minutes at 150 V (compared to 1 hour to overnight).
This new gel technology delivers uniform band shape and symmetry—lot-to-lot and intra-lot reproducibility ensure consistency of results.
“I think there’s a pervasive feeling among researchers that their own hand-poured gels have better resolution, but I would argue the opposite,” said Dr. Jensen. “The TGX gels provide a facile solution for producing publication-quality figures.”
Mini-Protean TGX gels demonstrate greater linearity of separation, which results in increased MW estimation accuracy when compared to traditional Laemmli precast gels or NuPAGE Bis-Tris gel systems. A linear regression analysis of estimated MW vs. known MW yields a coefficient of linearity (R2) of greater than 0.98 for TGX gels, vs. an R2 of only 0.86 for a representative NuPAGE gel.
Dynamic range of quantitation and resolution are also superior with TGX gel technology. TGX gels exhibit greater lane and band symmetry than traditional Laemmli gels or alternative long shelf life gels, even when overloaded.
The Mini-Protean TGX gels deliver superior staining with low background. These gels are compatible with all commonly used stains and mass spectrometry applications. Dr. Jensen remarked that using Bio-Rad’s precast gels prevented user contamination that could potentially interfere with mass spectrometric analysis when using handcast gels.
The Mini-Protean TGX precast gels provide transfer efficiency with both wet/tank and semi-dry transfer systems. The proteins from the gel can be transferred onto a PVDF or nitrocellulose membrane in as little as 15 minutes (Figure 2).