Before dispensing, the adherent HeLa S3 cells were examined under a microscope and were found to be viable, as seen in Figure 1A. After detachment with trypsin, the number of cells present were calculated and subsequently dispensed into microplates, again using the Multidrop Combi.
The cells in suspension were then observed under the microscope immediately after dispensing Figure 1B. These cells were viable and completely intact, with no visible damage. Following further cultivation, the cell culture adhered to the microplate where it continued to grow evenly—Figure 1C shows the cells 24 hours after adhering to the surface of a 96-well plate.
The cell proliferation assay demonstrated that the levels of viability for the cells dispensed using the Multidrop Combi and those dispensed with the manual pipette were the same. When using the standard tube-dispensing cassette, an even dispense is obtained across the 96-well plate, as seen in Figure 2A. The results shown are averages of all the wells in each column. Figure 2B demonstrates the same even distribution when using the small tube plastic tip dispensing cassette. Cell viability is comparable to hand pipetting with both cassettes and plates.